anti cd45 rabbit pab  (Servicebio Inc)

Journal: Journal of Translational Medicine

Article Title: Multi-omics analysis reveals a feedback loop amplifying immune responses in acute graft-versus-host disease due to imbalanced gut microbiota and bile acid metabolism

doi: 10.1186/s12967-024-05577-x

Figure Lengend Snippet: scRNA-seq analysis of PBMCs of patients who underwent allo-HSCT revealing immune regulation signature in aGVHD. A Unsupervised clustering (UMAP plot) of scRNA-seq data from PBMCs of patients with aGVHD and with non-aGVHD, with cell subtypes labeled. B The frequency percentage of the main cell subtypes (patients with non-aGVHD, n = 6; patients with aGVHD, n = 6). C Dot graph illustrating the GO analysis of upregulated genes in PBMCs of patients with non-aGVHD compared with those from individuals with aGVHD. D Dot plots illustrating relative expression levels of the upregulated genes IRF7 , IRF1 , NLRC4 , LILRB1 , LILRB2 , and LILRB4 in PBMCs of patients with non-aGVHD. E Dot graph illustrating the GO analysis of upregulated genes in PBMCs from patients with aGVHD compared with those from individuals with non-aGVHD. F Dot plots were used to display the relative expression levels of downregulated genes ( JUNB , FOS , JUN , CD83 , and EGR1 ) in PBMCs of patients with non-aGVHD. G Representative immunofluorescence staining of c-FOS, c-JUN, IRF7 and CD45 in small intestine of control or aGVHD mouse model groups. Scale bar, 20 µm

Article Snippet: The following primary antibodies were used: Anti-IRF7 Rabbit pAb (1:500, Cat# GB111169, Servicebio), Anti-c-Jun Rabbit pAb (1:500, Cat# GB11515, Servicebio), Anti-c-Fos Mouse mAb (1:1000, Cat# GB12069, Servicebio), Anti-CD45 Rabbit pAb (1:1000, Cat# GB113886, Servicebio), Anti-Resistin Rabbit pAb (1:5000, Cat# GB112526, Servicebio), and Anti-S100A9 Rabbit pAb (1:500, Cat# GB111149, Servicebio).

Techniques: Labeling, Expressing, Immunofluorescence, Staining, Control

Journal: Journal of Translational Medicine

Article Title: Multi-omics analysis reveals a feedback loop amplifying immune responses in acute graft-versus-host disease due to imbalanced gut microbiota and bile acid metabolism

doi: 10.1186/s12967-024-05577-x

Figure Lengend Snippet: Granulocyte-like cells derived from patients with non-aGVHD exhibited elevated levels of RETN expression, suggesting a potential role for resistin in preventing aGVHD. A Heatmap display of the communication probability of each cluster within the combined dataset of PBMCs from patients undergoing allo-HSCT. B RETN and its corresponding receptor CAP1 were expresed in granulocyte-like cells across each sample. C Representative immunofluorescence staining of CD45 and resistin in the small intestine of control or aGVHD mouse model groups. Scale bar, 100 µm. D IL-1β concentrations were measured using ELISA assay in cell culture supernatants (n = 3) following various treatments involving LPS and resistin. E Venn diagrams illustrating the shared conserved downregulated genes among distinct lineages (group 1: neutrophils vs. macrophages vs. monocytes; group 2: cDC1 vs. cDC2 vs. migratory DC; group 3: plasmacytoid dendritic cell (pDC) vs. B cells; group 4: CD4 + T cells vs. CD8 + T cells vs. Treg; group 5: innate lymphoid cell (ILC) vs. γδ T cells vs. NK cells). F Summary of the resistin-driven immune regulation in the presence of LPS or other special conditions

Article Snippet: The following primary antibodies were used: Anti-IRF7 Rabbit pAb (1:500, Cat# GB111169, Servicebio), Anti-c-Jun Rabbit pAb (1:500, Cat# GB11515, Servicebio), Anti-c-Fos Mouse mAb (1:1000, Cat# GB12069, Servicebio), Anti-CD45 Rabbit pAb (1:1000, Cat# GB113886, Servicebio), Anti-Resistin Rabbit pAb (1:5000, Cat# GB112526, Servicebio), and Anti-S100A9 Rabbit pAb (1:500, Cat# GB111149, Servicebio).

Techniques: Derivative Assay, Expressing, Immunofluorescence, Staining, Control, Enzyme-linked Immunosorbent Assay, Cell Culture

Journal: Journal of Translational Medicine

Article Title: Multi-omics analysis reveals a feedback loop amplifying immune responses in acute graft-versus-host disease due to imbalanced gut microbiota and bile acid metabolism

doi: 10.1186/s12967-024-05577-x

Figure Lengend Snippet: Relationship between GPBAR1 / NR1H2 expression and immune regulation in myeloid lineages through correlation analysis. A Volcano plots depicting the upregulated and downregulated DEGs in GPBAR1 and NR1H2 -expressing macrophage-like cells in comparison to their respective controls. B Volcano plots illustrating the upregulated and downregulated DEGs in GPBAR1 and NR1H2 -expressing CD14 − CD16 + monocytes compared to their respective controls. C Representative immunofluorescence staining of CD45 and S100A9 in the small intestine of control or aGVHD mouse model groups. Scale bar, 50 µm. D Relevance of GPBAR1/NR1H2 in immune regulation

Article Snippet: The following primary antibodies were used: Anti-IRF7 Rabbit pAb (1:500, Cat# GB111169, Servicebio), Anti-c-Jun Rabbit pAb (1:500, Cat# GB11515, Servicebio), Anti-c-Fos Mouse mAb (1:1000, Cat# GB12069, Servicebio), Anti-CD45 Rabbit pAb (1:1000, Cat# GB113886, Servicebio), Anti-Resistin Rabbit pAb (1:5000, Cat# GB112526, Servicebio), and Anti-S100A9 Rabbit pAb (1:500, Cat# GB111149, Servicebio).

Techniques: Expressing, Comparison, Immunofluorescence, Staining, Control